Nucleic Acid Chemistry Laboratory

A. Ono

Akira Ono
(Professor)

Office: 23-818 Ext: 3887

e-mail:  akiraono@kanagawa-u.ac.jp

1986, Ph.D., Hokkaido University

1983, M.S., Hokkaido University

1981, B.S., Hokkaido University

H. Saneyoshi

Hisao Saneyoshi
(Assistant Professor)

Laboratory: 23-826 Ext: 3901

e-mail: saneyoshih@kanagawa-u.ac.jp

Ph.D., Tokyo Institute of Technology

M.S., Tokyo Institute of Technology

B.S., Aoyama Gakuin University

Research Field

Bio-functional Chemistry and Engineering, Nucleic Acid Chemistry, Medicinal Chemistry, Bio-nano Chemistry.

Research Overview

Using skills in nucleic acids chemistry and synthetic chemistry, we are developing new materials and technologies to contribute in life and material sciences.

Research Subjects

  • 1.Development of oligonucleotide prodrugs.
  • 2.Synthesis, properties, and structures of metallo-DNA duplexes.
  • 3.Development of highly sensitive Hg(II) ion sensors and Hg(II) ion adsorbing materials.

Introduction:

Some of our recent results are shown below.

Nucleic Acid Chemotherapy

Mercury Ion Detection

Oligonucleotide-based therapies promise to be highly specific tools for treating numerous human diseases. However, the multi-anionic nature of oligonucleotides prohibits cellular internalization. In addition, oligonucleotides are sensitive to extra- and intracellular nucleolytic enzymes. To circumvent these drawbacks, we are developing oligonucleotide prodrugs (pro-oligos). Pro-oligos can penetrate cells, where the pro-oligos are activated by cellular functions.

Contamination with heavy metal ions may have severe effects on human health and the environment. Mercury contamination is widespread and arises from a variety of natural and anthropogenic sources. We are developing new methods for detecting and isolating mercury ions efficiently in aqueous solution.

Publications
  • 1)H. Saneyoshi*, K. Kondo, N. Sagawa, A. Ono*“Glutathione-triggered activation of the model of pro-oligonucleotide with benzyl protecting groups at the internucleotide linkage” Bioorg. Med. Chem. Lett., 2016, 26, 622-625.H.
  • 2)H. Saneyoshi,* Y. Hiyoshi, K. Iketani, K. Kondo, A. Ono* “Bioreductive deprotection of protected thymine bases in oligonucleotides for the activation of duplex formation” Bioorg. Med. Chem. Lett., 2015, 25, 5632-5635.
  • 3)H. Saneyoshi, Y. Hiyoshi, K. Iketani, K. Kondo, A. Ono, “Development of a photolabile protecting group for phosphodiesters in oligonucleotides,” Bioorg. Med. Chem. Lett., 2015, 25, 2129–2132.
  • 4)T. Kurokawa,* M. Igawa, A. Ono, and I. Okamoto* “Selective Transport of Mercury(II) Ions across Supported Liquid Membrane with Thymine Derivative as Carrier” Chem. Lett., 2015, 44, 1732–1734.
  • 5)Y. Tanaka* (4名) H. Saneyoshi, (2名) A. Ono* “Structures, physicochemical properties, and applications of T–HgII–T, C–AgI–C, and other metallo-base-pairs” Chem. Comm., 2015, 51, 17343-17360 (Feature Article).
Affiliated Academic Organizations

A. Ono:
The Chemical Society of Japan, The Pharmaceutical Society of Japan, The Society of Synthetic Organic Chemistry, Japan.

H. Saneyoshi:
The Chemical Society of Japan, The Japan Society of Analytical Chemistry.

Current members
◯ Professors: 1 ◯ Assistant Professors: 1
◯ Postgraduates (M.C.): 6 ◯ Undergraduates: 13

Facilities: DNA Synthesizer, high-performance liquid chromatography system, thermal melting system (TMSPC-8, Shimadzu) for measuring thermal denaturation profiles of nucleic acids, fluorescence spectrophotometer.

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