Plant Genetics and Breeding Laboratory

N. Asakura

Nobuaki Asakura

Office: 6-304 Ext: 3346


Ph.D., Kobe University

M.S., Kobe University

B.S., Kobe University

Research Field

Plant Genetics, Plant Breeding, Biological Sciences Education

Research Overview

Utilization of DNA markers for dissection of plant genetic diversity, enhancement of plant improvement, and development of educational materials for biological sciences.

Research Subjects

  • 1. Genetic studies on sea buckthorn (Hippophae rhamnoides L.) using DNA markers.
  • 2. Development of experimental programs to understand plant genetic diversity.
  • 3. Development of stable detection methods of transgenes in commercial processed foods including genetically modified crops.


Various techniques for DNA analyses have been developed from the end of the 20th century.  Technological innovation has still continued.  Many kinds of DNA markers have been utilized for studies of plant evolution and breeding.  We are interested in a dioecious plant, sea buckthorn (Hippophae rhamnoides L.) shown in Fig. 1 as a highly hopeful horticultural crop to human beings, because it includes a wide variety of nutrients.  Our studies using DNA markers have started to shed the light on the genetics of sea buckthorn.

  • 1. Kitchen PCR: An Experimental Programme to Experience Plant Genetic Diversity at the DNA Level. (2015) In: Daniel, E. G. S.(ed) Biology Education and Research in a Changing Planet., Springer, Singapore, pp.11-20.
  • 2. Comparative nucleotide sequence analysis of the D genome-specific sequence-tagged-site locus A1 in Triticum aestivum and its implication for the origin of subspecies sphaerococcum (2011) Breeding Science 61: 212-216.
  • 3. Genotyping of the Q locus in wheat by a simple PCR-RFLP method (2009) Genes & Genet. Syst., 84: 233-237.
  • 4. Sequence diversity and copy number variation of Mutator-like transposases in wheat. (2008) Genet. Mol. Biol., 31: 539-546.
  • 5. A transcriptionally active maize MuDR-like transposable element in rice and its relatives (2002) Mol. Genet. Genomics, 268: 321-330.
Affiliated Academic Organizations

Japanese Society of Breeding
The Genetic Society of Japan
Japanese Society for Horticultural Science
The Society of Biological Sciences Education of Japan

Current members
◯ Professors: 1 ◯ Undergraduates: 8

Facilities: DNA thermal cycler, Plant growth chamber, Clean bench.